LSM - Wide-ranging approaches in multi-color flow cytometry

LSM - Wide-ranging approaches in multi-color flow cytometry

“BD Horizon Tour”

Target audience: PhD students and Post-Docs that use flow cytometry in their research

Place: Jette campus, Faculty Meeting Room

Date/Time: 23rd January 2018 (9:00-15:00)

            Sandwich lunch provided.

Format:Instructor-led presentation,

Language of instruction: English

Max. participants: 20

Instructors:Roland Langelaar and Jean-Pierre Sepers,  application specialist in flowcytometry at the department of BD Life Sciences Benelux.

Programm: Click here for the full programm

LEARNING OBJECTIVES

By the end of this workshop you should be able to:

  • Know the characteristics of fluorescent dyes, their spectral spill over & compensation
  • Understand the fundamental concepts of panel design
  • Learn how to optimize your panel to achieve the best possible separation

CONTENT

As biology advances, the need for deep and broad profiling of cell populations is becoming ever more important. By delivering information from multiple parameters simultaneously, a single flow cytometry experiment can speed the path to discovery.

However, quality flow cytometry data starts with proper panel design.  In the Design module, we will walk through the three key considerations for panel design: understanding the biology of your experiment, characterizing your unique instrument and selecting the optimal fluorochromes.

In general, a good panel is defined by its ability to resolve populations of interest. Matching bright fluorochromes to dim markers, and the ability to spread markers across multiple lasers, can minimize spectral overlap and maximize population resolution. In the Achieve and Analyze module, you will learn the different considerations that apply when taking a panel from one instrument to another. In addition, we will show how experiments can be standardized across different flow cytometry platforms.

The Sort module will review important considerations when transitioning from cell analysis to cell sorting. Additionally, we will introduce the concept of index sorting and show how flow cytometry can prepare individual cells for downstream analysis.

Finally, the discover module will show you how flow cytometry is keeping pace with science through the ability to perform high parameter experiments.

REQUIRED SKILLS

Basic knowledge in flow cytometry is required

Registration:

Register here

Priority will be given to PhDs and Post-docs of the VUB, but others interested can apply as well.

We kindly ask you to register only once and only when you can actually participate. In case you have already registered but are not able to participate, please be so kind as to notify us by sending an e-mail to lsm [at] vub [dot] ac [dot] be so we can offer your place to one of your PhD/Post-doc colleagues on the waiting list.

Please be aware of the fact that the cancellation and no-show policy of VUB applies. Cancellation of your participation is only allowed up to 48 hours before the start of the course. Otherwise, the policy as described on the doctoral school website will be followed.

Registration Rules: https://student.vub.be/en/phd/doctoral-training#registration
No Show Policy: 
https://student.vub.be/en/regulations-and-forms#phd